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NAD+

Nicotinamide adenine dinucleotide, a ubiquitous coenzyme, not a peptide; precursor research targets age-related NAD decline.

Overview

NAD+ (nicotinamide adenine dinucleotide) is a fundamental coenzyme found in every cell, where it shuttles electrons in redox reactions and serves as a substrate for sirtuins, PARP enzymes, and other signaling proteins. It is a dinucleotide small molecule, not a peptide, but it appears in peptide catalogs because of intense research interest in the decline of NAD+ levels with age and in precursors such as nicotinamide riboside (NR) and nicotinamide mononucleotide (NMN).

Mechanism

NAD+ is required for ATP production through glycolysis and oxidative phosphorylation and is consumed by enzymes that regulate DNA repair, inflammation, and gene expression. The aging hypothesis holds that falling NAD+ availability impairs sirtuin activity and mitochondrial function; precursor supplementation is proposed to restore intracellular NAD+ pools.

Dosing

Human trials of the precursors NR and NMN have used a wide range of oral doses, generally from hundreds of milligrams to about one gram daily, with the specific regimens reported in each study. NAD+ itself is poorly absorbed orally and is mostly studied by intravenous infusion in early human protocols.

Expected Effects

  • reliable increases in blood NAD+ levels after precursor supplementation in human trials
  • improvements in some metabolic and mitochondrial markers in rodent studies
  • clinically meaningful anti-aging outcomes in humans remain unproven

Side Effects & Tolerability

Short-term trials of NR and NMN report good tolerability with mild gastrointestinal symptoms at higher doses. Long-term safety, especially regarding NAD-consuming pathways such as PARP and potential effects on tumor biology, is not yet established.

Storage

NAD+ and its precursors are moisture- and light-sensitive; research-grade material should be stored per the supplier label, typically frozen or refrigerated, desiccated, and protected from light.

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